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Pluro – Best IVF & Fertility Clinic Across India

Personalised fertility care from a compassionate, outcomes-focused team. Advanced technology, transparent pricing, and end-to-end support for your parenthood journey.

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Office No. 101-107, 1st Floor, One 45 Business Bay, Vallabh Baug Ln Ext, Railway Police Colony, Ghatkopar East, Mumbai, Maharashtra - 400 075
022 3522 6216
contact@pluro.in

© 2026 Pluro Fertility and IVF

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Pluro – Best IVF & Fertility Clinic Across India

Personalised fertility care from a compassionate, outcomes-focused team. Advanced technology, transparent pricing, and end-to-end support for your parenthood journey.

Our StoryTreatmentsOur DoctorsFind ClinicsAcademyContact UsBlogs

© 2026 Pluro Fertility and IVF

Privacy PolicyTerms of ServiceRefunds & CancellationsSitemap
    how-eggs-are-fertilised-ivf-laboratory

    IVF & Treatment Basics

    How are eggs fertilised in the IVF laboratory?

    After egg retrieval, everything happens in our embryology lab, where conditions are carefully controlled to closely mimic your body and support optimal embryo developme

    April 17, 2026
    2 min read

    Standard IVF Insemination

    Conventional IVF involves mixing washed and concentrated sperm in a dish along with the eggs, where they naturally fertilise the eggs during overnight incubation. In IVF, the presence of two pronuclei in the egg (2PN) the following morning confirms fertilisation. IVF is usually performed when sperm parameters are satisfactory.

    ICSI: When a Single Sperm Is Injected

    ICSI, in contrast, entails the introduction of one sperm cell per egg with the help of micromanipulation techniques involving a microscopic needle. The application of ICSI is recommended if there are few sperms, low sperm motility or sperm morphology, repeated fertilization failure using conventional IVF techniques, and surgical sperms (PESA/TESA). At Pluro, our embryologists select the best individual sperms using high magnification microscopy.

    Days 1–5: From Fertilised Egg to Blastocyst

    Day 1: Fertilisation confirmed. Days 2–3: Embryo divides to 4–8 cells (cleavage stage). Day 5: The best embryos reach blastocyst stage, a 100+ cell structure with a defined inner cell mass (future baby) and outer trophectoderm (future placenta). Pluro waits until Day 5 because blastocysts have higher implantation rates and allow AI-assisted grading to identify the strongest embryo.

    Frequently Asked Questions

    Q: How will I know how my embryos are developing?

    A: Your team will call with updates on Day 1 (fertilisation), Day 3 (cleavage), and Day 5 (blastocyst). You can also ask at any point.

    Q: What is Time-Lapse Monitoring?

    A: Some centres use time-lapse incubators (such as Embryoscope) that photograph embryos every few minutes without removing them from incubation. This gives embryologists more data to select the best embryo. Ask if this is available at your clinic. Such type of traditional way of embryo selection has equivalent results as compare to Embryoscope.

    Q: Are there risks to ICSI that standard IVF does not have?

    A:ICSI eliminates the natural process of sperm selection. However, theoretically, this procedure may pose some increased risks of genetic diseases. The results, however, in practice show that these are comparable for most people. Your physician will advise you about this possibility, if applicable.


    Frequently Asked Questions

    How will I know how my embryos are developing?

    Your team will call with updates on Day 1 (fertilisation), Day 3 (cleavage), and Day 5 (blastocyst). You can also ask at any point

    What is Time-Lapse Monitoring?

    Some centres use time-lapse incubators (such as Embryoscope) that photograph embryos every few minutes without removing them from incubation. This gives embryologists more data to select the best embryo. Ask if this is available at your clinic. Such type of traditional way of embryo selection has equivalent results as compare to Embryoscope

    Are there risks to ICSI that standard IVF does not have?

    ICSI eliminates the natural process of sperm selection. However, theoretically, this procedure may pose some increased risks of genetic diseases. The results, however, in practice show that these are comparable for most people. Your physician will advise you about this possibility, if applicable.

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